BVDA - Crowle's stain

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SHOEPRINTS

SHOEPRINTS IN BLOOD

Crowle's stain

Crowle's Stain, also a protein stain like for example Acid Violet 17 and Amido Black, colors marks in blood red. It is also known as Crowle's double stain because it contains two dyes: Crocein Scarlet 7B (major component) and Coomassie Brilliant Blue R (minor component).

The formulation contains a toxic substance (trichloroacetic acid) and acetic acid but is otherwise water-based.

The stained marks can hardly be lifted with a white gelatin lifter and do not fluoresce, either on the treated surface or the gellifter. In comparisons Crowle's stain gives less contrast than Amido Black or Acid Violet 17.

Shoeprint in blood on PVC flooring, cut in four and stained with different reagents (clockwise from top left: Amido Black, Crowle's stain, Coomassie Blue, and Hungarian Red).

The part of the shoeprint in blood on PVC flooring that was stained with Crowle's stain.

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B-88500

Crowle's Stain, 500 ml in a spray bottle

Name: Crocein scarlet 7B, CI Acid Red 71, 1-Naphthalenesulfonic acid,7-hydroxy-8-[2-[2-methyl-4-[2-(2-methyl-4-sulfophenyl)diazenyl]phenyl]diazenyl]-, sodium salt (1:2)
CAS No.: 6226-76-2

Name: Coomassie Brilliant Blue R, CI Acid Blue 83, Benzenemethanaminium, N-(4-((4-((4-ethoxyphenyl)amino)phenyl)(4-(ethyl((3-sulfophenyl)methyl)amino)phenyl)methylene)-2,5-cyclohexadien-1-ylidene)-N-ethyl-3-sulfo-, inner salt, sodium salt (1:1)
CAS No.: 6104-59-2